杭州招聘

1. 根据蛋白表达项目要求，对项目进行文献查找和分析，能够较清晰地建立研发路径，对每个关键节点进行把控和难点问题进行分析解决，并能够主导建立研发管理流程。熟悉大肠杆菌、毕氏酵母、真核细胞或昆虫细胞中两种或两种以上开发表达工艺者优先。（研发主管需满足该要求）；

2.在公司资源引导下主导抗体噬菌体表面展示技术平台、抗体酵母表面展示技术的建立和工艺优化；

3.承担分子生物相关实验工作：包括但不限于PCR、酶切、载体构建、蛋白诱导表达、DNA/RNA/质粒提取等；

4. 质粒生产制备：主要包括质粒大抽、浓缩、除菌等实验室常规的工作、执行并优化现有流程；

5. 承担杂交瘤细胞或单个B细胞的抗体可变基因扩增钓取、PCR产物测序，并且进行抗体表达载体的构建和性能确认。

6.其他与重组蛋白表达分析相关的工作。

岗位要求：

1. 生物工程、细胞生物学、基因工程等生物或药学相关专业，硕士以上学历；

2. 2年以上工作经历，有扎实的微生物学、基因工程及细胞学知识，优秀的无菌细胞操作能力；

3. 熟悉哺乳动物表达系统，做过多个重组蛋白瞬时表达；

4. 良好的沟通能力和团队合作精神，有多系统蛋白表达经验者优先。

Job description：

1. According to the requirements of protein expression project, search relevant documents and analysis the project， can clearly establish the R & D path, well control each key node analyze and solve the difficulties, can lead to establish R & D management process. Skilled with two or more expression technologies in Bacillus coli, Pichia pastoris, eukaryotic cells or insect cells is preferred. (the R & D director shall meet this requirement);

2.Lead the establishment of antibody phage and antibody yeast surface display technology and process optimization;

3. Undertake molecular biology related experiments, including but not limited to PCR, enzyme digestion, vector construction, protein induced expression, DNA / RNA / plasmid extraction, etc;

4. Preparation for plasmid production : mainly include plasmid extraction, concentration and sterilization. Implement and optimize the existing process;

5. To amplify and fish variable genes of antibody from hybridoma cells or single B cells, sequence PCR products, and construct antibody expression vector and confirm its performance.

6. Other work related to analysis of recombinant protein expression .

Job requirements:

1. Master's degree or above in bioengineering, cell biology, genetic engineering, etc;

2. More than 2 years working experience, solid knowledge of Microbiology, genetic engineering and cytology, excellent aseptic cell operation ability;

3. Be familiar with mammalian expression system，have experience of several transient expression of recombinant proteins;

4. Good communication skills and teamwork spirit, multi system protein expression experience is preferred.

印月生物技术（杭州）有限公司是一家新兴的高科技生物技术公司，座落于美丽的杭州市西湖区，取名印月。公司拥有成熟的杂交瘤抗体发现平台，噬菌体展示抗体平台和B单细胞克隆抗体平台。团队成员对诊断和治疗性抗体方面具有多年的经验，并取得一定成就。公司秉承着“严谨专致，突破创新”的服务理念，致力于开发具有高临床价值和市场前景的核心生物原材料，为国内外客户提供快速有效的一站式抗体定制服务。
Yinyue Biotechnology (Hangzhou) Co., Ltd. is a new developing high-tech biotechnology company, located in the beautiful West Lake District of Hangzhou City, named Yinyue. The company has mature hybridoma antibody discovery platform, phage display antibody platform and B single cell clone antibody platform. Our team has many years of experience in diagnosis and treatment of antibodies, and got some significant achievements. Adhering to the service concept of "rigorous focus, breakthrough and innovation", the company is committed to the development of core biological raw materials with high clinical value and market prospect, and provides rapid and effective one-stop antibody customization service for domestic and foreign customers.