北京招聘

  1.   General job accountability:  

• Perform total RNA extraction and RT-PCR:

  • Mouse single B cell suspension preparation;

  • Extract high quality total RNA by Trizol reagent;

  • Conduct first strand cDNA synthesis.

• Perform antibody phage libraries construction:

  • Conduct the first round PCR and gel purification of 1st PCR product and sequence quality analysis;

  • Conduct and optimize the second overlap PCR and sequence quality analysis;

  • Prepare high transformation efficiency electro-competent cells;

  • Be responsible for phage library packing and purification.

• Perform antibody phage libraries panning and screening:

  • Perform QC of antigen/biotinylated antigen;

  • Conduct phage solid, liquid and cell panning;

  • Conduct colony picking and culture and induction;

  • Perform high-throughput ELSIA or mirrorball screening;

  • Re-array positive isolates and sequencing;

  • Perform unique sequence analysis and characterization.

• Coordinate lead isolation characterization:

  • Closely work with biology team and support antibody discovery;

• Keep appropriate experimental records and documentation:

                                          2.   Qualification and competency requirements：  

  

A. Education/training background:

  

• Master with 2+ years, or BS with 5+ years of experience Molecular Biology, Biochemistry, cell biology etc.;

• At least 2 years working experience in pharmaceutical or biotech companies is required.

    

  

B. Knowledge/Skills:

  

• Mastery of molecular biology(e.g. PCR, RT-PCR, molecular cloning etc.).

• Hands-on experience in phage display technology.

• Knowledge of antibody properties, structure and sequences is required.

• Knowledge of molecular biology or protein chemistry or structure biology is plus.

• Experience of therapeutic antibody is plus.

• Excellent data management is plus.

• Excellent presentation skills is plus.

• Experience of antibody repertoire analysis is plus.

• Experience of protein structure modeling is plus.

• Experience of following software / tools is plus:

  • Molecular biology: VectorNTI, SnapGene, CLC Workbench, etc.,

  • Data analysis and process: Origin, GraphPad Prism, FlowJo, Spotfire, etc..

                        

C. Others:

  

• Good planning and execution skills to meet deadlines and to handle multi tasks.

• Quick learner and good team player.

• Strong problem-solving skills.

      

和铂医药是一家专注于肿瘤免疫领域创新药物研究与开发的全球化运营生物医药公司。公司将运用其拥有的全部自主知识产权的全人源单抗的转基因鼠系列核心技术平台，建立下一代治疗性肿瘤抗体药物产品管线,并将围绕Harbour的两大平台拓展合作与授权业务。公司总部及研发基地位于中国上海，另外在美国马塞诸塞州剑桥设立商业运营中心，在荷兰鹿特丹设立抗体研发平台创新中心，在波士顿地区设立了创新中心包括实验室和研发团队，也将在江苏昆山设立研发基地。公司拥有资深管理团队以及丰富的药物研究与开发经验。和铂医药要为满足中国及全球病人的需求而不断努力，依托拥有自主知识产权的核心技术平台,创建一个在肿瘤治疗领域有科学与技术领导力的国际性生物医药公司。同时, 在药物研发领域建立以技术平台为基础的对外合作，整合新的技术，推动中国整个抗体研发产业的进步。

Company Overview
Harbour BioMed is a global biotechnology company discovering and developing innovative therapeutics for cancer with a focus on immuno-oncology and autoimmunity/inflammation. The company's discovery and development programs are centered around its two patented transgenic mouse platforms for human antibody discovery.
The company is building its proprietary pipeline internally, through collaborations with co-development partners, as well as through selective preclinical and clinical stage asset acquisitions. Harbour BioMed also licenses the platforms to companies and academic institutions through its Harbour Antibodies subsidiary.
Harbour BioMed has headquarters and an R&D site in Shanghai, business operations in Cambridge, MA, and antibody platform development in Rotterdam, The Netherlands.
The company has an innovation center including research lab and a development team in Boston. Also, we are expanding to Kunshan site in Jiangsu for our R&D operation.